QuestionJuly 11, 2025

The parameter (s) that affects the amount of light absorbed by a sample in passing through a spectrophotometer is (are): The thickness of the solution through which the light passes. The concentration of the absorbing species in solution. The absorptivity coefficient of the absorbing species. All of the above.

The parameter (s) that affects the amount of light absorbed by a sample in passing through a spectrophotometer is (are): The thickness of the solution through which the light passes. The concentration of the absorbing species in solution. The absorptivity coefficient of the absorbing species. All of the above.
The parameter (s) that affects the amount of light absorbed by a sample in passing
through a spectrophotometer is (are):
The thickness of the solution through which the light passes.
The concentration of the absorbing species in solution.
The absorptivity coefficient of the absorbing species.
All of the above.

Solution
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Answer

All of the above. Explanation 1. Identify Parameters Affecting Absorption The amount of light absorbed by a sample in a spectrophotometer is governed by **Beer-Lambert Law**: A = \epsilon \cdot c \cdot l, where A is absorbance, \epsilon is the absorptivity coefficient, c is the concentration, and l is the path length (thickness). 2. Analyze Each Parameter All listed parameters—thickness (path length), concentration, and absorptivity coefficient—are part of the Beer-Lambert Law equation and thus affect absorption.

Explanation

1. Identify Parameters Affecting Absorption<br /> The amount of light absorbed by a sample in a spectrophotometer is governed by **Beer-Lambert Law**: $A = \epsilon \cdot c \cdot l$, where $A$ is absorbance, $\epsilon$ is the absorptivity coefficient, $c$ is the concentration, and $l$ is the path length (thickness).<br /><br />2. Analyze Each Parameter<br /> All listed parameters—thickness (path length), concentration, and absorptivity coefficient—are part of the Beer-Lambert Law equation and thus affect absorption.
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